Generator

Part:BBa_K2447500

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-11)


Constituitive expression of Photosensitive EL222 bacterial protein

This part allows constitutive production of the light-sensitive bacterial photosensitive protein EL222, a blue light activated transcription factor. This part could prove useful in the design of optogenetic (blue-light) circuit to regulate protein expression.

BBa_K2447500 is a basic part, a sensor. It has been characterized using Blue-light inducible system with RFP reporter (BBa_K2447501)

Experiment results showed that the part is working. The results are shown under Experience.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 551
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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